ETF1 Rabbit pAb for WB, IF/ICC, ELISA - P62495

Core Product Specifications and Parameters

Biological Background: ETF1 Function and Localization

• ETF1 (Eukaryotic peptide chain release factor subunit 1), also known as ERF1, RF1, or SUP45L1, is a key factor in protein synthesis.
• Functions within the eRF1-eRF3-GTP ternary complex to recognize stop codons and catalyze peptidyl-tRNA hydrolysis, leading to polypeptide chain release (PubMed:10676813, PubMed:16777602, PubMed:24486019, PubMed:26245381, PubMed:27863242, PubMed:36638793). Related references: PMID:10676813, PMID:16777602, PMID:24486019, PMID:26245381, PMID:27863242, PMID:36638793
• Component of the transient SURF complex that recruits UPF1 to stalled ribosomes, linking translation termination to nonsense-mediated mRNA decay (NMD) (PubMed:19417104). Related references: PMID:19417104
• Required for SHFL-mediated translation termination that suppresses programmed −1 ribosomal frameshifting (−1PRF) on viral and cellular mRNAs (PubMed:30682371). Related references: PMID:30682371
• Predominantly localized in the cytoplasm.
• Subject to post-translational modifications including acetylation, methylation, hydroxylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 278–437 in the C-terminal region of human ETF1; verify reactivity against full-length protein and any isoforms in your sample system.
• For western blotting, a band around 49 kDa is expected; optimize loading and use appropriate positive controls from human, mouse, or rat cell lysates.
• In immunofluorescence/ICC, expect cytoplasmic staining; include negative controls to confirm specificity.
• For ELISA, the antibody may be tested as a capture or detection reagent; consider pairing with a suitable matched antibody for sandwich assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research manufacturers with integrated access to high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. The ETF1 antibody supports research into translation termination and nonsense-mediated decay pathways. Our sourcing team can also assist with hard-to-find targets and custom reagent procurement to streamline your workflow from concept to clinic.