ESRP1 Rabbit Polyclonal Antibody for WB, ELISA - Q6NXG1

Core Product Specifications and Parameters

Biological Background: ESRP1 Function and Localization

• ESRP1 (Epithelial splicing regulatory protein 1), also known as RBM35A, is an RNA-binding protein that functions as a splicing factor, primarily regulating the formation of epithelial cell-specific isoforms.
• It specifically controls the splicing of FGFR2 to produce the FGFR2-IIIb isoform, an epithelial-specific variant, by binding to GU-rich sequence motifs in the ISE/ISS-3 cis-element region of the FGFR2 pre-mRNA (PubMed:19285943). Related references: PMID:19285943
• ESRP1 also regulates alternative splicing of CD44, CTNND1 (p120-catenin), and ENAH, three transcripts that undergo splicing changes during the epithelial-to-mesenchymal transition (EMT).
• Subcellular localization is predominantly nuclear, consistent with its role in pre-mRNA processing and splicing regulation.
• It is expressed specifically in epithelial cells, aligning with its function in maintaining epithelial cell-specific splicing programs.
• Post-translational modifications include phosphorylation and methylation, which may modulate its activity or interactions.
• According to sequence homology studies, ESRP1 is implicated in inner ear development, auditory hair cell differentiation, and cell fate specification in the cochlear epithelium (By similarity).
• Mutations in ESRP1 are associated with non-syndromic deafness (DFNB109), highlighting its importance in auditory function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-220) of human ESRP1; therefore, the antibody may recognize both full-length and any N-terminal isoforms or degradation products.
• For Western blot (WB), consider using epithelial cell lines (e.g., HeLa, MCF-7) or mouse epithelial tissues as positive controls, given the epithelial-specific expression of ESRP1.
• In ELISA applications, direct coating with the recombinant immunogen or native protein from cell lysates can be explored; however, optimal antibody dilution and detection conditions should be determined experimentally.
• Due to cross-reactivity with mouse ESRP1, this antibody can be used for comparative studies in mouse models, but validation in the specific mouse tissue or cell type is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, providing easy access to validated antibodies, recombinant proteins, and ancillary reagents for diagnostic development. This ESRP1 rabbit polyclonal antibody, targeting a key epithelial splicing regulator, supports research into EMT and deafness mechanisms. Contact us for bulk orders, custom sourcing, or technical consulting to accelerate your project from concept to clinic.