ERAL1 Rabbit Polyclonal Antibody for WB and ELISA - O75616

Core Product Specifications and Parameters

Biological Background: ERAL1 Function and Localization

• ERAL1, also known as GTPase Era mitochondrial or conserved ERA-like GTPase (CEGA), is a member of the Era family of GTP-binding proteins.
• It functions as a probable GTPase critical for mitochondrial ribosomal small subunit assembly, specifically binding to a 33-nucleotide section of the 12S mitochondrial rRNA at its 3′ terminal stem-loop region.
• ERAL1 may act as a molecular chaperone that protects the 12S mt-rRNA on the 28S mitoribosomal subunit during assembly of the small subunit, ensuring proper ribosome biogenesis.
• The protein localizes to the mitochondrial matrix and inner membrane, consistent with its role in mitoribosome maturation.
• Post-translational modifications include phosphorylation (phosphoprotein), which may regulate its activity.
• Mutations in ERAL1 are associated with deafness and other disease variants, highlighting its importance in mitochondrial function.
• The human ERAL1 gene undergoes alternative splicing, producing multiple isoforms such as ERA-W and HERA-A/B.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 198–437) of human ERAL1, which includes the GTPase domain; consider this when analyzing isoform reactivity.
• For WB, ERAL1 has a predicted molecular weight of 48 kDa; use appropriate gel percentage and transfer conditions.
• As a polyclonal antibody, it may exhibit batch-to-batch variation; validation in the relevant sample system (e.g., mitochondrial lysates) is recommended.
• For ELISA, the antibody may be used as a capture or detection reagent after optimizing coating and blocking conditions.
• Indicated species cross-reactivity with mouse and rat should be confirmed by sequence alignment of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For ERAL1-related mitochondrial research and assay development, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your workflow. Partner with CamelBio to accelerate your next-generation diagnostic solutions.