ERAB/HSD17B10 Rabbit Monoclonal Antibody for WB, ELISA - Q99714

Core Product Specifications and Parameters

Biological Background: ERAB/HSD17B10 (3-hydroxyacyl-CoA dehydrogenase type-2) Function and Localization

• Multifunctional mitochondrial enzyme with dual roles: acts as an (S)-3-hydroxyacyl-CoA dehydrogenase in fatty acid beta-oxidation and also catalyzes oxidation of 3-hydroxy-2-methylbutyryl-CoA in isoleucine degradation. Prefers short- and medium-chain substrates with highest efficiency for (3S)-hydroxybutanoyl-CoA. Related references: PMID:10600649, PMID:12917011, PMID:18996107
• Moonlights as a non-catalytic subunit (MRPP2) of the mitochondrial ribonuclease P complex, involved in tRNA 5'-end cleavage, N1-methylation of guanine and adenine at position 9 in tRNAs, and serving as a tRNA maturation platform to facilitate 3'-processing and CCA addition. Related references: PMID:18984158, PMID:23042678, PMID:24549042, PMID:29040705
• Exhibits hydroxysteroid dehydrogenase activity, oxidizing 3alpha-, 17beta-, 20beta- and 21-hydroxysteroids as well as 7alpha- and 7beta-hydroxy bile acids. Notably oxidizes allopregnanolone/brexanolone at the 3alpha-hydroxyl group, which may impact GABA-A receptor activation. Related references: PMID:19706438, PMID:28888424
• Possesses phospholipase C-like activity toward cardiolipin and its oxidized species, likely degrading damaged cardiolipin during oxidative stress to protect cells from apoptosis. Related references: PMID:26338420
• Interacts with intracellular amyloid-beta, which may contribute to neuronal dysfunction in Alzheimer disease (AD); overexpression observed in AD-affected neurons. Related references: PMID:9338779
• Subcellular localization: predominantly mitochondrial matrix and associated with mitochondrial nucleoids, essential for mitochondrial structural and functional integrity. Related references: PMID:20077426
• Post-translational modifications include acetylation, and genetic variants are linked to HSD10 mitochondrial disease (intellectual disability and neurodegeneration).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 162–261 near the C-terminus. Consider using a blocking peptide to verify specific band detection in WB.
• Based on its molecular weight (27kDa), ensure adequate gel separation in the 20–35kDa range and transfer conditions optimized for small proteins.
• WB: validate antibody performance in your target species (human, mouse, rat) using appropriate tissue or cell lysates known to express HSD17B10 (e.g., mitochondria-enriched fractions).
• ELISA: a direct or indirect ELISA with recombinant antigen or peptide coating may help assess batch-to-batch consistency.
• For applications involving post-translational modifications or interaction studies, consider that acetylation and amyloid-beta binding may influence epitope accessibility.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For neurodegenerative disease and metabolic research, CamelBio can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and supporting ancillary reagents like this HSD17B10 monoclonal antibody. Our rare target raw-material sourcing capability ensures you can secure the exact tools needed for robust assay development.