ENPEP Rabbit pAb for WB, IHC-P, ELISA - Q07075

Core Product Specifications and Parameters

Biological Background: Glutamyl aminopeptidase Function and Localization

• Glutamyl aminopeptidase (ENPEP), also known as aminopeptidase A (AP-A) or gp160, is a transmembrane metalloprotease.
• It functions in blood pressure regulation by cleaving N-terminal acidic residues, particularly from angiotensin II, in a calcium-modulated manner.
• The enzyme belongs to the M1 family of aminopeptidases and binds zinc as a cofactor for its catalytic activity.
• ENPEP is localized to the cell membrane, anchored by a signal-anchor sequence.
• Tissue expression includes choriocarcinoma cancer cell lines (at protein level), epithelial cells of renal proximal tubules and glomerulus, and various other tissues. Related references: PMID:10692253
• It is a glycoprotein with disulfide bonds and contains a transmembrane helix.
• The molecular weight of the full-length protein is approximately 109 kDa.

Experimental Guidance and Technical Tips

• For Western blot analysis, use reducing SDS-PAGE conditions and ensure thorough denaturation to account for the protein's disulfide bonds.
• The predicted molecular weight is 109 kDa; verify band migration in the relevant cell or tissue lysate.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), consider heat-induced antigen retrieval methods to expose the epitope.
• In ELISA applications, confirm optimal coating concentration and detection conditions using recombinant or native ENPEP.
• The antibody cross-reacts with human, mouse, and rat; validate specificity in the target species and tissue background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw material procurement by providing validated antibodies, including this ENPEP polyclonal, to support research in hypertension and renal biology. We offer comprehensive solutions—from optimized monoclonal/polyclonal antibodies to bulk ancillary reagents—ensuring reliable sourcing even for rare targets like aminopeptidase A.