ENPEP Rabbit pAb for WB, ELISA - Q07075

Core Product Specifications and Parameters

Biological Background: Glutamyl Aminopeptidase (ENPEP) Function and Localization

• ENPEP encodes glutamyl aminopeptidase (aminopeptidase A, APA), a zinc-dependent metalloprotease and type II integral membrane glycoprotein.
• It specifically hydrolyzes N-terminal acidic residues (glutamate and aspartate) from peptides like angiotensin II, thereby regulating the renin-angiotensin system and central hypertension.
• Calcium ions modulate enzyme activity, enhancing substrate cleavage efficiency.
• Expressed on the cell membrane of renal proximal tubule and glomerular epithelial cells, and in choriocarcinoma cell lines (at protein level). Related references: PMID:10692253
• Structural studies reveal a homodimeric architecture with a catalytic zinc ion coordinated by conserved residues; the protein also contains disulfide bonds and N-linked glycosylation.
• Known as CD249 or gp160, it was originally characterized as a differentiation antigen on certain cell types.
• Additional post-translational modifications include signal-anchor processing and transmembrane helix anchoring to the cell surface.

Experimental Guidance and Technical Tips

• The immunogen lies within amino acids 850-950 of the C-terminal intracellular domain; consider permeabilization for applications like flow cytometry.
• For Western blot, the predicted molecular weight is approximately 109 kDa; optimize gel percentage and transfer conditions accordingly.
• In ELISA assays, verify cross-reactivity with mouse ENPEP due to sequence homology in the epitope region.
• Validate antibody performance in relevant cell lines (e.g., renal proximal tubule cells) or tissues known to express ENPEP.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD antibodies and raw materials, including validated anti-ENPEP antibodies for hypertension and renal disease research. Our one-stop sourcing platform combines custom reagent development, bulk supply logistics, and technical consulting to accelerate your diagnostic assay development from concept to clinic.