eNOS Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P29474

Core Product Specifications and Parameters

Biological Background: eNOS Function and Localization

• Produces nitric oxide (NO), a key signaling molecule that induces vascular smooth muscle relaxation via cGMP-mediated signal transduction, essential for blood pressure regulation. Related references: PMID:1378832
• Mediates VEGF-induced angiogenesis in coronary vessels and promotes blood clotting through platelet activation.
• A naturally occurring splice variant lacks eNOS activity and may down-regulate functional eNOS by forming inactive heterodimers.
• Primarily localizes to the plasma membrane and caveolae, but also found in the cytoplasm, associated with the cytoskeleton, and in the Golgi apparatus.
• Highly expressed in platelets, placenta, liver, and kidney, reflecting its roles in vascular homeostasis and platelet function.
• Regulated by multiple post-translational modifications including phosphorylation, myristoylation, and palmitoylation, and requires cofactors such as FAD, FMN, heme, and zinc for activity.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 1000-1203) of human eNOS, which may enable detection of both full-length and truncated variants; consider verifying specificity in relevant cell models expressing eNOS.
• For Western blot, a band migrating at approximately 133 kDa is expected. Use broad-range molecular weight markers and optimize sample preparation (e.g., using reducing conditions) for clear results.
• In immunofluorescence (paraffin-embedded samples), antigen retrieval protocols may be necessary; pilot testing with known positive controls (e.g., human umbilical vein endothelial cells) is recommended.
• The antibody cross-reacts with mouse and rat eNOS due to sequence conservation; validate with appropriate tissue or cell lysates from these species before quantitative comparisons.
• ELISA applications may require checkerboard titrations to determine optimal coating antigen and antibody concentrations; the antibody may be used for capture or detection in sandwich immunoassays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive raw-material partner for IVD manufacturers and research laboratories, offering validated antibodies, bulk ancillary reagents, and rare target sourcing. For researchers focusing on nitric oxide signaling and vascular biology, this anti-eNOS polyclonal antibody provides a reliable tool for multiple applications. Contact our team to discuss customized antibody pair sourcing, bulk supply, or technical support for your assay development pipeline.