ENO2 Rabbit Polyclonal Antibody for WB and ELISA - P09104

Core Product Specifications and Parameters

Biological Background: ENO2 Function and Localization

• Gamma-enolase, also known as neuron-specific enolase (NSE) or ENO2, is a glycolytic enzyme that catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.
• It plays a role in both glycolysis and gluconeogenesis, mediating energy metabolism.
• Beyond its metabolic function, ENO2 exhibits neurotrophic and neuroprotective properties, promoting survival of central nervous system neurons.
• The protein localizes primarily to the cytoplasm and can also be found at the cell membrane.
• ENO2 shows tissue-specific expression: the alpha/gamma heterodimer and gamma/gamma homodimer are predominantly found in neurons, while other enolase isoforms are expressed in muscle and other tissues.
• Post-translational modifications include acetylation, phosphorylation, hydroxylation, and ubiquitination, as indicated by proteomics studies.
• The gene undergoes alternative splicing and the protein features isopeptide bond formation and metal binding (magnesium).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-127); consider this when validating specificity in downstream applications.
• For WB, a band around 47 kDa is expected; use appropriate loading controls and optimize blocking to minimize non-specific binding.
• For ELISA, the antibody may recognize denatured epitopes; evaluation under native conditions is recommended if required.
• With confirmed cross-reactivity to mouse and rat, this antibody is suitable for studies in rodent models; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with streamlined access to high-quality IVD antibodies, including this ENO2 polyclonal antibody for glycolysis and neurological disease research. Our comprehensive portfolio includes validated antibody pairs, recombinant proteins, and bulk ancillary reagents to accelerate assay development. From rare target sourcing to custom production, CamelBio supports your entire R&D workflow.