ENO2 Rabbit mAb for WB - P09104

Core Product Specifications and Parameters

Biological Background: Gamma-enolase Function and Localization

• Gamma-enolase (ENO2), also known as neuron-specific enolase (NSE) or 2-phospho-D-glycerate hydro-lyase, is one of three enolase isoenzymes found in mammals. The gene ENO2 encodes this glycolytic enzyme.
• It catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis, central to cellular energy metabolism.
• Beyond its metabolic role, gamma-enolase exhibits neurotrophic and neuroprotective functions on a broad spectrum of central nervous system neurons, binding to neocortical neurons in a calcium-dependent manner and promoting cell survival.
• The protein is predominantly expressed as homodimers (gamma/gamma) and heterodimers (alpha/gamma) in neurons, while other enolase dimers are distributed in various tissues: alpha/alpha in most adult tissues, alpha/beta and beta/beta in striated muscle.
• Subcellularly, gamma-enolase localizes to the cytoplasm and can associate with the cell membrane.
• Post-translational modifications include acetylation, phosphorylation, hydroxylation, and ubiquitin-like conjugation, as identified by proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen for this rabbit monoclonal antibody is a recombinant fusion protein spanning amino acids 47-100 of human ENO2 (NP_001966.1), a region conserved across human, mouse, and rat, consistent with the reported cross-reactivity.
• For Western blotting, a band at approximately 47 kDa is expected under reducing conditions; ensure optimal sample preparation and loading controls.
• In immunofluorescence (IF/ICC and IF-F) and immunohistochemistry (IHC-P), consider antigen retrieval methods and appropriate fixation/permeabilization protocols, as ENO2 localizes to the cytoplasm and membrane.
• For ELISA applications, this antibody may be used for capture or detection, but pairing with a validated antibody pair is recommended for sandwich ELISA development.
• As ENO2 (NSE) is a clinical biomarker for neuroendocrine tumors and neuronal damage, validate the antibody in relevant sample matrices (e.g., serum, cell lysates) using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers, laboratories, and research institutes as a comprehensive IVD raw-material sourcing partner. The ENO2 Rabbit mAb exemplifies our offering of high-quality monoclonal antibodies for neuroscience and oncology biomarker research. Beyond individual antibodies, we supply validated antibody pairs, optimized bulk reagents, and assist with rare-target procurement to streamline your assay development from concept to clinic.