ENO1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P06733

Core Product Specifications and Parameters

Biological Background: Alpha-enolase (ENO1) Function and Localization

• Alpha-enolase (ENO1) is a multifunctional protein that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. Related references: PMID:1369209, PMID:29775581
• It is involved in growth control, hypoxia tolerance, and allergic responses. Related references: PMID:10802057, PMID:12666133, PMID:2005901
• On the cell surface, ENO1 functions as a plasminogen receptor and activator, contributing to the fibrinolytic system. Related references: PMID:12666133
• It stimulates immunoglobulin production. Related references: PMID:1369209
• ENO1 also acts as a transcriptional repressor by binding to the myc promoter and may possess tumor suppressor activity.
• Subcellular localization includes the cytoplasm, cell membrane, nucleus, and the M line of sarcomeres in striated muscle.
• The alpha/alpha homodimer is widely expressed in most tissues, whereas tissue-specific heterodimers with beta or gamma enolases are found in muscle and neurons, respectively.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitination regulate its functions, and it features DNA-binding and transcriptional regulatory activities.

Experimental Guidance and Technical Tips

• For Western blot (WB), use reducing SDS-PAGE with whole-cell lysates from human or mouse cells; a band approximately 47 kDa should be detected.
• In immunohistochemistry (IHC-P), antigen retrieval methods may be required; optimize citrate or EDTA buffer conditions for each tissue type.
• For immunofluorescence (IF/ICC), fixation and permeabilization protocols can affect the detection of ENO1 due to its presence in multiple subcellular compartments; include appropriate controls.
• In immunoprecipitation (IP), the polyclonal nature of this antibody may provide efficient capture of native protein complexes; validate via Western blot.
• For ELISA, this antibody can be used as capture or detection reagent after confirming compatibility with coating buffers and detection systems.
• All applications should include relevant positive and negative control samples; cross-reactivity with mouse allows for use in mouse-derived models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and researchers, offering one-stop sourcing of IVD raw materials, including validated antibodies for key metabolic and cancer-related targets like alpha-enolase. Our supply capabilities encompass optimized antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and rare target sourcing to support your assay development from concept to clinic. Whether you require antibody customization, technical consultation, or scalable raw material procurement, CamelBio provides integrated solutions to accelerate your projects.