ELOVL6 Rabbit Polyclonal Antibody for WB, ELISA - Q9H5J4

Core Product Specifications and Parameters

Biological Background: ELOVL6 Function and Localization

• ELOVL6 (Very long chain fatty acid elongase 6) is a member of the ELOVL family of fatty acid elongases.
• It catalyzes the first and rate-limiting step in the elongation cycle of long-chain fatty acids, condensing C12, C14, and C16 acyl-CoAs, with highest activity toward C16:0.
• This ER membrane-bound enzyme contributes to the synthesis of unsaturated C16 long chain fatty acids and, to a lesser extent, C18:0 and those with low desaturation degree.
• It may participate in the production of saturated and monounsaturated very long chain fatty acids (VLCFAs) that serve as precursors for membrane lipids and lipid mediators.
• Subcellular localization is at the endoplasmic reticulum membrane.
• Expression is ubiquitous, suggesting a fundamental role in cellular lipid metabolism.
• According to UniProt keywords, ELOVL6 is a glycoprotein, transferase, multi-pass transmembrane protein, and is involved in fatty acid biosynthesis and metabolism.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a synthetic peptide spanning amino acids 50–150 of human ELOVL6. This region is predicted to be intracellular, potentially accessible in denaturing conditions for Western blotting.
• Application considerations: For Western blot, consider using denaturing and reducing conditions to expose the epitope. Validate in relevant sample systems expressing ELOVL6, such as human, mouse, or rat tissues/cells.
• For ELISA, the polyclonal nature may provide high sensitivity, but potential cross-reactivity with other ELOVL family members should be assessed.
• Protein size: The predicted molecular weight is 31 kDa, but the observed band may differ due to glycosylation or other post-translational modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials and technical support. For studies involving ELOVL6 and fatty acid metabolism, we offer validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. Our sourcing capabilities include rare target raw materials, enabling streamlined procurement from initial research to clinical application.