ELMOD3 Rabbit Polyclonal Antibody for WB, ELISA - Q96FG2

Core Product Specifications and Parameters

Biological Background: ELMOD3 Function and Localization

• ELMOD3 (ELMO domain-containing protein 3) is also known as RBED1, RBM29, LST3, and is linked to deafness loci DFNA81 and DFNB88.
• Functions as a GTPase-activating protein (GAP) for ARL2, though with low specific activity.
• Localizes to cell projections including stereocilia and kinocilia, as well as the cytoplasm and cytoskeleton.
• Both isoform 1 and isoform 6 are widely expressed across tissues.
• Predicted molecular weight is approximately 43 kDa, consistent with observed band sizes.
• Mutations in ELMOD3 cause autosomal dominant and recessive non-syndromic hearing loss.
• Contains ELMO and RNA-binding motif domains, suggesting potential roles in RNA processing and signal transduction.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 290–381, which may be present in both major isoforms; verify isoform specificity in your model system.
• For Western blot, anticipate a specific band near 43 kDa in lysates from human, mouse, and rat tissues; optimization of protein load and blocking conditions is advised.
• In ELISA, using the immunogen recombinant protein as a positive control can help establish assay sensitivity and dynamic range.
• As a polyclonal, lot-to-lot consistency should be monitored; we recommend routine quality checks with appropriate reference standards.
• Consider epitope exposure and retrieval methods for immunohistochemical applications, as localization is cytoskeletal and in specialized cell protrusions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. This ELMOD3 antibody expands our portfolio of thoroughly characterized immunoglobulin reagents for GTPase signaling and hereditary deafness research. Beyond primary antibodies, we supply validated antibody pairs, recombinant proteins, and bulk ancillary reagents—empowering seamless integration into your diagnostic and research workflows.