EIF6 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P56537

Core Product Specifications and Parameters

Biological Background: Eukaryotic Translation Initiation Factor 6 (EIF6) Function and Localization

• EIF6 (also known as p27BBP, ITGB4BP, CAB) is a eukaryotic translation initiation factor that regulates ribosomal subunit association.
• Binds to the 60S ribosomal subunit and prevents its premature assembly with the 40S subunit in the cytoplasm, thereby inhibiting 80S initiation complex formation. Acts as a stimulatory translation initiation factor downstream of insulin and growth factor signaling. Related references: PMID:10085284 PMID:14654845 PMID:21536732
• Involved in ribosome biogenesis by associating with pre-60S subunits in the nucleus and facilitating their nuclear export. Related references: PMID:10085284 PMID:14654845 PMID:21536732
• Functions in miRNA-mediated gene silencing through the RNA-induced silencing complex (RISC), being required for both translational repression and target mRNA cleavage. Related references: PMID:17507929
• In insulin-responsive tissues, controls fatty acid synthesis and glycolysis by modulating the translation of adipogenic transcription factors (CEBPB, CEBPD, ATF4) containing G/C-rich or uORF motifs in their 5' UTR.
• Required for reactive oxygen species (ROS)-dependent megakaryocyte maturation and platelet formation by regulating mitochondrial respiratory chain gene expression.
• Localizes to the cytoplasm, nucleus, and nucleolus, reflecting its dual roles in translation initiation and ribosome biogenesis.
• Highly expressed in colon carcinoma at the protein level, with lower levels in normal colon, ileum, kidney, and muscle.
• Classified under translation initiation factors, and annotated with keywords including phosphoprotein, Ubl conjugation, and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal 245 amino acids of human EIF6; the resulting polyclonal antibody may recognize full-length EIF6 as well as potential isoforms.
• For Western blotting, the predicted molecular weight is 27kDa; validate band specificity using appropriate positive and negative controls (e.g., EIF6-overexpressing or knockdown cell lysates).
• In immunofluorescence/ICC experiments, expect both cytoplasmic and nucleolar staining due to EIF6's dual localization; optimize fixation and permeabilization conditions for your cell type.
• Cross-reactivity with mouse and rat is indicated; however, confirm reactivity in your specific sample system, as signal intensity may vary.
• For ELISA applications, determine the optimal antibody dilution and coating antigen concentration by checkerboard titration.
• Consider that EIF6 involvement in miRNA silencing and translation control may require siRNA or compound treatments to observe dynamic changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering dependable access to EIF6 antibodies and related reagents for research and diagnostic development. From validated antibody pairs to optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, we support your workflow from concept to clinic. Whether your focus is translational regulation in cancer or ribosome biogenesis, CamelBio streamlines procurement to meet your critical raw-material needs.