EIF4G2/p97 Rabbit mAb for WB, IHC-P, IP, and ELISA - P78344

Core Product Specifications and Parameters

Biological Background: EIF4G2/p97 Function and Localization

• EIF4G2 (Eukaryotic translation initiation factor 4 gamma 2), also known as p97, DAP-5 (Death-associated protein 5), and NAT1, is a member of the eIF4G family involved in translation initiation.
• It functions as an adapter that plays a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis, and viral infection.
• The protein is cleaved by some caspases and viral proteases, generating fragments that modulate translation initiation.
• Ubiquitously expressed in adult tissues, with highest levels observed in skeletal muscle and heart; also expressed in fetal brain, lung, liver, and kidney.
• EIF4G2 is subject to multiple post-translational modifications including acetylation, methylation, and phosphorylation, as well as ubiquitination.
• Alternative splicing of the EIF4G2 gene yields multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning amino acids 320-490; consider this region when evaluating isoform coverage.
• For western blot, a band at approximately 102 kDa is expected under reducing conditions.
• For immunohistochemistry, validate in tissue sections expressing high levels of EIF4G2, such as skeletal muscle or heart.
• As a monoclonal antibody, lot-to-lot consistency is expected, but individual optimization of dilution factors and conditions is recommended.

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