eIF4A1 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P60842

Core Product Specifications and Parameters

Biological Background: eIF4A1 Function and Localization

• eIF4A1 is an ATP-dependent RNA helicase and a core component of the eIF4F translation initiation complex, recognizing the mRNA cap structure and facilitating ribosome binding.
• It unwinds RNA secondary structures in the 5'-UTR of mRNAs, enabling efficient scanning by the small ribosomal subunit for the initiator codon and thereby driving cap-dependent translation initiation. Related references: PMID:20156963
• Through its helicase activity, eIF4A1 promotes cell proliferation and growth, acting as a key regulator of gene expression at the translational level.
• eIF4A1 localizes to the cytoplasm, perinuclear region, and cell membrane, and can also be found in stress granules under cellular stress conditions.
• The protein undergoes various post-translational modifications, including acetylation, phosphorylation, and ubiquitination, which may modulate its activity and interactions.
• Encoded by the EIF4A1 gene (also known as DDX2A or EIF4A), alternative splicing produces multiple isoforms of this conserved DEAD-box helicase.
• eIF4A1 participates in host-virus interactions, as numerous viruses hijack the host translation machinery for their own protein synthesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human eIF4A1, so the antibody may specifically recognize the N-terminal epitope; confirm specificity in your experimental system.
• For Western blotting, expect a band near 46kDa (UniProt calculated molecular weight 46.2kDa), though actual migration may vary depending on gel composition and sample preparation.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization conditions to detect eIF4A1 in its cytoplasmic and membrane-associated pools.
• The antibody is reported to cross-react with mouse, rat, and monkey eIF4A1; always validate reactivity and signal-to-noise ratio in samples from these species before large-scale studies.
• For immunoprecipitation (IP) and ELISA, consider using fresh or properly stored lysates to preserve protein interactions and conformation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to provide a seamless supply of high-quality IVD raw materials. This anti-eIF4A1 monoclonal antibody supports research into translation initiation mechanisms and their roles in cancer and viral infections. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing services for rare-target raw materials, helping you move from concept to clinic efficiently.