EIF3H Rabbit Polyclonal Antibody for WB, IP, IF/ICC, ELISA - O15372

Core Product Specifications and Parameters

Biological Background: EIF3H Function and Localization

• EIF3H (eIF3S3, eIF3-p40, eIF3-gamma) is a subunit of the eukaryotic translation initiation factor 3 (eIF-3) complex, which plays a critical role in the initiation of protein synthesis.
• The eIF-3 complex associates with the 40S ribosomal subunit and facilitates the recruitment of other initiation factors to form the 43S pre-initiation complex (43S PIC), stimulating mRNA recruitment and scanning for AUG recognition. Related references: PMID:17581632
• EIF3H is involved in the disassembly and recycling of post-termination ribosomal complexes and prevents premature joining of ribosomal subunits prior to initiation. Related references: PMID:17581632
• The eIF-3 complex specifically targets and regulates translation of mRNAs involved in cell proliferation, including cell cycling, differentiation, and apoptosis, using different RNA stem-loop binding modes. Related references: PMID:25849773 PMID:27462815
• EIF3H is localized in the cytoplasm.
• Post-translational modifications of EIF3H include phosphorylation and isopeptide bond formation.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein encompassing amino acids 80-340 of human EIF3H, which may contribute to the antibody's specificity.
• For Western blotting, a band around 40 kDa is expected based on the predicted protein weight; use appropriate controls to confirm specific detection.
• This antibody is reported to work in WB, IP, IF/ICC, and ELISA; for immunoprecipitation and immunofluorescence, optimization of antibody concentration and fixation conditions is advised.
• Cross-reactivity is noted for human, mouse, and rat, but we recommend verifying performance in each species and application of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, technical services, and consulting to support diagnostic manufacturers, labs, and research institutes from concept to clinical application. This EIF3H rabbit polyclonal antibody is part of our translation initiation and protein synthesis research portfolio. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare target raw materials to streamline your procurement needs.