EIF3F Rabbit Polyclonal Antibody (pAb) for WB, IHC-P, IF/ICC, IP, ELISA - O00303

Core Product Specifications and Parameters

Biological Background: EIF3F Function and Localization

• EIF3F (also known as eIF3-p47, EIF3S5, MRT67) is a core subunit of the eukaryotic translation initiation factor 3 (eIF-3) complex.
• The eIF-3 complex is required for multiple steps in protein synthesis initiation, including recruitment of initiation factors to the 40S ribosome, stimulation of mRNA recruitment and scanning, and disassembly and recycling of post-termination ribosomal complexes.
  Related references: PMID:17581632, PMID:25849773, PMID:27462815
• EIF3F possesses deubiquitinating enzyme (thiol protease) activity and specifically deubiquitinates activated NOTCH1, promoting its nuclear import and thereby acting as a positive regulator of Notch signaling.
• Post-translational modifications of EIF3F include acetylation, phosphorylation, and ubiquitination.
• The protein predominantly localizes to the cytoplasm.
• Mutations in EIF3F have been linked to intellectual disability.
• Predicted molecular weight is approximately 37.6 kDa, with observed migration at 38 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen was designed as a recombinant fusion protein covering amino acids 88-357 of human EIF3F (NP_003745.1). This region may detect full-length EIF3F and potentially some isoforms.
• For Western blotting, the predicted band size is approximately 38 kDa. Consider using appropriate positive controls (e.g., HeLa, HEK293 cell lysates) based on known expression.
• For immunohistochemistry (IHC-P), antigen retrieval and detection systems may need optimization for each tissue and fixation method.
• In immunofluorescence (IF/ICC), EIF3F shows cytoplasmic localization; counterstaining with DAPI is recommended.
• For immunoprecipitation (IP), use a cross-reactive species‑matched lysate and verify binding efficiency.
• ELISA performance may depend on coating buffer and detection modality; consider pairing with a suitable secondary antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For research on translation initiation and protein synthesis pathways, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials. This EIF3F rabbit polyclonal antibody is part of our comprehensive portfolio to support your assay development needs.