EIF2S3 Rabbit Polyclonal Antibody for WB, ELISA - P41091

Core Product Specifications and Parameters

Biological Background: EIF2S3 Function and Localization

• EIF2S3 encodes the gamma subunit of eukaryotic translation initiation factor 2 (eIF2), which is essential for protein synthesis initiation. eIF2 forms a ternary complex with GTP and initiator tRNA, which then binds to the 40S ribosomal subunit to form the 43S pre-initiation complex (PubMed:31836389). Related references: PMID:31836389
• The eIF2 complex is a heterotrimer consisting of alpha, beta, and gamma subunits; the gamma subunit (EIF2S3) binds GTP and is responsible for hydrolyzing GTP upon formation of the 80S initiation complex, leading to release of eIF2-GDP.
• EIF2S3 is located in the cytoplasm, specifically in the cytosol, where translation initiation occurs.
• The protein is expressed in multiple tissues, including testis, brain, liver, and muscle, indicating its fundamental role in protein biosynthesis across different cell types.
• EIF2S3 is subject to post-translational modifications, including acetylation and phosphorylation, which may regulate its activity and interaction with other initiation factors.
• Mutations in EIF2S3 are associated with diseases such as MEHMO syndrome (mental retardation, epileptic seizures, hypogonadism, microcephaly, obesity), highlighting its critical role in neurological development and function.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using whole cell lysates from human or rat cell lines, as the antibody cross-reacts with rat. The predicted molecular weight is 51 kDa; include appropriate molecular weight markers and verify band specificity.
• For ELISA, the recombinant immunogen (amino acids 173-472 of human EIF2S3) can serve as a positive control to assess antibody binding. Optimize coating concentrations and detection conditions.
• Since this is a polyclonal antibody, lot-to-lot variability may occur. Validate each new lot in your specific assay system and consider using a blocking buffer containing non-fat milk or BSA.
• For applications beyond WB and ELISA, pilot experiments are recommended to determine suitability. Note that the antibody has been validated for WB and ELISA; other applications require further testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers, labs, and research institutes, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For EIF2S3 research in translation initiation and protein synthesis, we can support your project with a complete range of complementary reagents, from concept to clinic. Leverage our one-stop access to accelerate your development workflow with reliable, traceable materials.