EHD4 Rabbit pAb for WB and ELISA - Q9H223

Core Product Specifications and Parameters

Biological Background: EHD4 Function and Localization

• EHD4 (EH domain-containing protein 4), also referred to as PAST4 or hepatocellular carcinoma-associated protein 10/11, is a member of the EHD ATPase family involved in intracellular membrane trafficking.
• It acts as an ATP- and membrane-binding protein that orchestrates membrane reorganization and tubulation upon ATP hydrolysis, playing a critical role in early endosomal transport (PubMed:17233914, PubMed:18331452). Related references: PMID:17233914 PMID:18331452
• During sprouting angiogenesis, EHD4 forms a complex with PACSIN2 and MICALL1 to generate recycling endosome-like tubular structures at asymmetric adherens junctions, thereby regulating CDH5 trafficking.
• Subcellularly, EHD4 is associated with early endosome and recycling endosome membranes, and it also localizes to the cell membrane and adherens junctions.
• EHD4 is highly expressed in pancreas and heart, indicating tissue-specific functional roles.
• The protein undergoes acetylation and phosphorylation, and it features ATP-binding and calcium-binding motifs that support its regulatory activities in membrane dynamics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 362–541 (C-terminal region) of human EHD4; verify that this region is conserved in your target species if using non‑human/mouse samples.
• For Western blot, a band near 61 kDa is expected; consider using positive control lysates from EHD4‑expressing tissues such as pancreas or heart.
• In ELISA, use a recombinant EHD4 fragment or full‑length protein as a standard and titrate the antibody to determine optimal coating and detection conditions.
• As a polyclonal antibody, evaluate lot‑to‑lot consistency for reproducibility in quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, supporting projects from early development to clinical application. This EHD4 antibody supports studies in endosomal trafficking, angiogenesis, and related disease areas such as cancer and cardiovascular disorders. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare‑target raw materials to streamline your assay development workflow.