EGFR Rabbit Polyclonal Antibody for WB, IP, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGFR Function and Localization

• EGFR is a receptor tyrosine kinase and member of the ErbB family (ErbB-1/HER1). The EGFR gene encodes multiple isoforms; isoform 2 may act as an antagonist of EGF action.
• Ligand binding: EGFR binds EGF family ligands, including EGF, TGF-alpha, AREG, epigen, BTC, epiregulin, and HBEGF, triggering receptor homo- and heterodimerization and autophosphorylation (PubMed:10805725, PubMed:27153536, PubMed:2790960, PubMed:12297049, PubMed:15611079, PubMed:17909029). Related references: PMID:10805725, PMID:27153536, PMID:2790960, PMID:12297049, PMID:15611079, PMID:17909029
• Activated EGFR recruits adapter proteins and signals through major cascades: RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, STATs, and potentially NF-kappa-B (PubMed:27153536, PubMed:11116146). Related references: PMID:27153536, PMID:11116146
• EGFR directly phosphorylates other proteins, such as RGS16 (PubMed:11602604) and MUC1 (PubMed:11483589), linking to G protein-coupled receptor signaling and beta-catenin pathways. Related references: PMID:11602604, PMID:11483589
• It regulates cell migration via interaction with CCDC88A/GIV (PubMed:20462955) and plays roles in learning, memory, and pain signaling (By similarity). Related references: PMID:20462955
• Subcellular localization: cell membrane, endoplasmic reticulum membrane, Golgi apparatus membrane, nucleus membrane, endosome membrane, endosome, nucleus, and also secreted.
• Ubiquitously expressed; isoform 2 is additionally expressed in ovarian cancers.
• Acts as a receptor for hepatitis C virus (HCV) entry, mediating hepatocyte infection.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal peptide (aa 1100–1200) of human EGFR; the antibody is expected to recognize full-length EGFR and potentially major isoforms.
• For Western blotting, use a positive control such as A431 cell lysate; the predicted molecular weight is ~134 kDa, but the glycoprotein may migrate larger due to post‑translational modifications.
• Consider using phosphatase inhibitors during sample preparation to preserve phosphorylation status, as EGFR is heavily phosphorylated upon activation.
• For immunoprecipitation, validate the antibody‑bead conjugate and optimize washing conditions to reduce background.
• Cross‑reactivity with mouse and rat has been demonstrated; however, we recommend verifying reactivity in the specific experimental model.
• Since this is a polyclonal antibody, batch‑to‑batch consistency should be assessed for critical quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting. This EGFR polyclonal antibody supports oncology, receptor signaling, and virology studies, and we can also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with sourcing rare‑target raw materials to streamline your diagnostic development from concept to clinic.