EGFR Rabbit mAb for WB, IHC-P, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: Epidermal Growth Factor Receptor Function and Localization

• EGFR, also known as ErbB-1 or HER1, is a receptor tyrosine kinase that binds various EGF family ligands, including EGF, TGF-alpha, and betacellulin, to activate intracellular signaling (PubMed:12297049, PubMed:15611079, PubMed:17909029). Related references: PMID:12297049 PMID:15611079 PMID:17909029
• Upon ligand binding, EGFR dimerizes and autophosphorylates, triggering major downstream pathways such as RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT modules (PubMed:27153536). Related references: PMID:27153536
• EGFR also activates NF-κB signaling and directly phosphorylates proteins like RGS16 and MUC1 to modulate GTPase activity and cell adhesion (PubMed:11116146, PubMed:11602604, PubMed:11483589). Related references: PMID:11116146 PMID:11602604 PMID:11483589
• Isoform 2 acts as an EGF antagonist and is expressed in ovarian cancers, while the main isoform is ubiquitously expressed in tissues.
• Subcellularly, EGFR localizes to the cell membrane, endoplasmic reticulum, Golgi, endosomes, and nucleus, reflecting its dynamic trafficking.
• EGFR serves as a host receptor for hepatitis C virus entry into hepatocytes by promoting CD81-CLDN1 complex formation.
• The protein undergoes various post-translational modifications such as phosphorylation, ubiquitination, and palmitoylation, and is involved in development and disease.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 335-525 in the extracellular domain of human EGFR, potentially enabling recognition of both membrane-bound and secreted forms.
• For Western blot (WB), note that EGFR has multiple reported molecular weights (44, 69, 77, and 134 kDa) due to glycosylation and alternative isoforms; validate with proper controls in your specific sample system.
• In immunohistochemistry (IHC-P), antigen retrieval and antibody dilution may require optimization based on tissue type and fixation.
• As a rabbit monoclonal antibody, it may provide high affinity and reproducibility for ELISA applications; however, assay conditions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, including high-quality antibodies like this anti-EGFR clone, supporting cancer biomarker development and diagnostic manufacturing. Our portfolio spans validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents, with expertise in rare target sourcing to accelerate your pipeline from concept to clinic.