EGF Receptor (E746-A750del Specific) Rabbit Polyclonal Antibody for WB, ELISA - P00533

Core Product Specifications and Parameters

Biological Background: EGF Receptor (EGFR) Function and Localization

• EGFR is a receptor tyrosine kinase that binds multiple EGF family ligands (e.g., EGF, TGFA, AREG, epigen, BTC, EREG, HBEGF). Ligand binding triggers homo‑/heterodimerization, autophosphorylation, and activation of downstream signaling pathways including RAS-RAF-MEK-ERK, PI3K-AKT, PLCgamma-PKC, STATs, and NF‑kappa‑B. This antibody is specific for the E746‑A750 deletion mutant, which is commonly found in non‑small cell lung cancer. Related references: PMID:10805725 PMID:27153536 PMID:2790960 PMID:12297049 PMID:15611079 PMID:17909029 PMID:11116146 PMID:11602604 PMID:11483589 PMID:20462955
• Isoform 2 of EGFR may act as an antagonist of EGF action, while the canonical isoform 1 is the predominant signaling receptor.
• EGFR is localized at the cell membrane, but also resides in the endoplasmic reticulum, Golgi apparatus, nucleus, endosomes, and can be secreted.
• The protein is extensively post‑translationally modified, including phosphorylation, glycosylation, hydroxylation, palmitoylation, methylation, and ubiquitination, which regulate its activity and trafficking.
• It is ubiquitously expressed, with isoform 2 additionally found in ovarian cancers.
• EGFR also serves as a host cell receptor for hepatitis C virus (HCV) entry, mediating CD81-CLDN1 complex formation and membrane fusion.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein encompassing the E746-A750 deletion, indicating that the antibody is designed to selectively recognize the mutant epitope. Experimenters should confirm specificity using wild‑type EGFR‑expressing cells as a negative control.
• For Western blotting, consider using protein lysates from human cancer cell lines known to harbor the EGFR E746‑A750del mutation (e.g., HCC827, H1975) or recombinant mutant EGFR protein.
• For ELISA, coating with recombinant E746‑A750del EGFR protein is advised. Recognize that cross‑reactivity with wild‑type EGFR may occur; appropriate blocking and dilution optimization are necessary.
• Optimal working concentrations for WB and ELISA should be determined empirically; typical starting dilutions for polyclonal antibodies range from 1:500 to 1:2000.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material partner, offering diagnostic manufacturers and researchers streamlined access to validated antibodies, ancillary reagents, and custom sourcing—from discovery to clinical application. This EGFR (E746‑A750del) polyclonal antibody supports oncology research and liquid biopsy assay development. For bulk orders, matched antibody pairs, or rare‑target sourcing, CamelBio provides responsive B2B support to accelerate your project timelines.