EEFSEC Rabbit pAb - P57772

Core Product Specifications and Parameters

Biological Background: Selenocysteine-specific elongation factor Function and Localization

• EEFSEC (SELB) is a translation factor required for the incorporation of the rare amino acid selenocysteine (Sec) at UGA codons, which normally signal termination. It replaces the eRF1-eRF3-GTP complex to enable selenocysteine insertion. Related references: PMID:27708257, PMID:35709277
• The selenocysteine incorporation process is mediated by SECISBP2 and EEFSEC, where SECISBP2 binds the SECIS element in selenoprotein mRNAs and GTP-bound EEFSEC delivers selenocysteyl-tRNA(Sec) to the ribosome. Related references: PMID:35709277
• Alternative splicing of EEFSEC produces multiple isoforms, expanding functional diversity.
• EEFSEC localizes to both the cytoplasm and the nucleus, consistent with its roles in translation and potential nuclear functions.
• Post-translational modifications, including phosphorylation and methylation, may regulate EEFSEC activity and interactions.
• Disease-associated variants in EEFSEC are linked to intellectual disability and neurodegeneration, highlighting its importance in neuronal function.
• The calculated molecular weight of EEFSEC is approximately 65 kDa, consistent with the observed band in Western blot.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 347–596 of human EEFSEC; thus, the antibody may recognize epitopes in the C‑terminal region. Consider validating reactivity in your specific experimental context.
• For Western blot, the expected band is around 65 kDa. Use appropriate molecular weight markers and loading controls; verify specificity in human cell lines or tissues expressing EEFSEC.
• For ELISA, ensure that the coating antigen matches the immunogen sequence for optimal detection. Pairing with a detection antibody targeting a different epitope may improve assay sensitivity.
• The antibody is supplied as polyclonal; lot‑to‑lot consistency should be confirmed if used in quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research laboratories with comprehensive access to IVD raw materials, from validated antibodies and recombinant proteins to ancillary reagents. For selenoprotein research and EFLSEC‑related projects, we can source polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk reagents tailored to your workflow. Our expertise in rare target sourcing and technical consulting streamlines your path from concept to clinic.