ECH1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q13011

Core Product Specifications and Parameters

Biological Background: ECH1 Function and Localization

• ECH1 encodes delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, a mitochondrial and peroxisomal enzyme belonging to the hydratase/isomerase superfamily.
• It catalyzes the isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, a critical step in the beta-oxidation of unsaturated fatty acids with double bonds at odd positions. Related references: PMID:26474213
• Together with 2,4-dienoyl-CoA reductase (DECR) and enoyl-CoA delta isomerase 1 (ECI1), ECH1 enables re-entry of unsaturated enoyl-CoA esters into the beta-oxidation cycle. Related references: PMID:26474213
• Subcellular localization studies indicate ECH1 is present in both the mitochondrion and peroxisome, supporting its role in fatty acid degradation.
• Post-translational modifications such as acetylation and phosphorylation have been identified on ECH1, potentially regulating its activity.
• Based on its catalytic mechanism, ECH1 is predicted to act on a broader range of fatty acid substrates beyond those experimentally tested.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human ECH1 protein (amino acids 1–328), which may allow recognition of both native and denatured forms; suitable for WB, IHC-P, and ELISA applications as listed.
• For Western blot, the predicted molecular weight is approximately 36 kDa; validate performance in the relevant cell or tissue lysate, and consider using loading controls from matching species.
• Cross-reactivity with mouse and rat ECH1 has been listed; sequence homology suggests likely recognition, but confirm in the corresponding model system prior to use.
• For IHC-P, optimize antigen retrieval conditions and antibody dilution; include appropriate positive and negative tissue controls to assess specificity.
• Since ECH1 localizes to mitochondria and peroxisomes, subcellular fractionation may enhance detection in complex samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing for fatty acid metabolism and mitochondrial research. For your ECH1 studies, CamelBio can support your procurement needs with reliable supply and expert guidance.