EBF1 Rabbit pAb for WB, ELISA - Q9UH73

Core Product Specifications and Parameters

Biological Background: EBF1 Function and Localization

• Encoded by the EBF1 gene, transcription factor COE1 (Early B-cell factor) is a member of the COE family and contains a zinc-finger DNA-binding domain.
• Key pioneer transcription factor of B-cell specification and commitment (PubMed:27807034). Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Related references: PMID:27807034
• Operates in a transcription factor network to activate B-cell-specific genes (e.g., BCR, CD40) while repressing genes associated with alternative cell fates, such as GATA3 and TCF7 for the T-cell lineage.
• Controls the thermogenic gene program in adipocytes during development and in response to environmental cold (By similarity).
• During Epstein-Barr virus infection, acts as a chromatin anchor for EBNA2 to mediate assembly of EBNA2 chromatin complexes (PubMed:28968461) and binds to the viral LMP1 proximal promoter to promote its expression during latency (PubMed:26819314). Related references: PMID:28968461 PMID:26819314
• Localizes to the nucleus, consistent with its role as a transcription factor.
• Subject to post-translational modifications including acetylation and ubiquitin-like conjugation, indicating regulation by multiple cellular signals.
• Alternatively spliced isoforms may exist, expanding functional diversity (based on UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 500-591 in the C-terminal region of human EBF1, which may confer target specificity.
• For WB, expect a band around 64 kDa. Validate using nuclear extracts from human B-cell lines (e.g., Ramos, Raji) or relevant primary tissues.
• The antibody is validated for ELISA; consider optimizing coating and detection protocols for your specific assay format.
• As a polyclonal antibody, confirm batch-to-batch consistency when used in critical or regulated applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutes with one-stop access to premium IVD raw materials, including antibodies like this EBF1 rabbit pAb. Whether you need validated antibody pairs for ELISA kits, bulk ancillary reagents, or rare-target sourcing for B-cell biology and transcription factor research, our team supports your project from concept to clinic. Count on us as your reliable partner for high-performance IVD components.