E2F6 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA, ChIP - O75461

Core Product Specifications and Parameters

Biological Background: Transcription factor E2F6 Function and Localization

• Transcriptional repressor: E2F6 acts as an inhibitor of E2F-dependent transcription. It binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3', with a preference for 5'-TTTCCCGC-3'. Unlike activator E2Fs, E2F6 lacks transcriptional activation and pocket protein binding domains. Related references: PMID:9501179, PMID:9704927
• Cell cycle regulation: E2F6 regulates a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. Overexpression delays the exit of cells from S-phase. Related references: PMID:9501179, PMID:9689056
• Repression mechanism: It may silence gene expression by recruiting a chromatin remodeling complex that contains histone H3-K9 methyltransferase activity.
• Meiosis-specific gene repression: E2F6 represses expression of some meiosis-specific genes, including SLC25A31/ANT4 (by similarity).
• Subcellular localization: The protein is primarily localized to the nucleus.
• Tissue expression pattern: Expressed in all tissues examined, with highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine, and spleen.
• Post-translational modifications: Subject to ubiquitination (Ubl conjugation) and alternative splicing, generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–281) of human E2F6, which is conserved across known isoforms. Consider verifying isoform specificity if required for your experimental context.
• Predicted molecular weight is 32 kDa; however, endogenous E2F6 may appear at slightly different sizes due to post-translational modifications. Always validate the band pattern in your sample system.
• For ChIP applications, while the antibody is raised against a recombinant protein, we recommend performing a ChIP-grade validation in your specific cell type and target locus.
• Cross-reactivity with mouse indicates epitope conservation, making this antibody a candidate for mouse model studies. Confirm reactivity and specificity in mouse-derived samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For studies on cell cycle control and transcriptional regulation, we facilitate procurement of rare-target raw materials and provide expert technical consulting to help advance your assay development from concept to clinic.