E2F6 Rabbit Monoclonal Antibody for WB, ChIP, CUT&Tag, ELISA - O75461

Core Product Specifications and Parameters

Biological Background: E2F6 Function and Localization

• Transcription factor E2F6 acts as an inhibitor of E2F-dependent transcription, lacking transcriptional activation and pocket protein binding domains. Related references: PMID:9501179, PMID:9689056, PMID:9704927
• Binds DNA cooperatively with DP proteins through the E2 recognition site, with a preference for the 5'-TTTCCCGC-3' motif. Related references: PMID:9501179
• Regulates a subset of E2F-dependent genes required for entry into the cell cycle; overexpression delays S-phase exit. Related references: PMID:9501179, PMID:9689056
• Represses expression of some meiosis-specific genes, including SLC25A31/ANT4, possibly via recruitment of a chromatin remodeling complex with histone H3-K9 methyltransferase activity.
• Localizes to the nucleus.
• Expressed in all tissues examined, with highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine, and spleen; undergoes alternative splicing and is subject to ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 110–281 of human E2F6, covering the central region of the protein; consider epitope accessibility in your assay format.
• As a monoclonal antibody, it is suitable for detection in Western blot (predicted band ~32 kDa), and may perform in chromatin immunoprecipitation (ChIP) and CUT&Tag due to its DNA-binding target; validate under your specific experimental conditions.
• For ELISA and other applications, optimize antibody concentration and blocking conditions using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For E2F6 and related transcription/cell cycle targets, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Let us streamline your procurement from discovery to commercial application.