E2F1 Rabbit Polyclonal Antibody for WB and ELISA - Q01094

Core Product Specifications and Parameters

Biological Background: E2F1 Function and Localization

• E2F1 (Transcription factor E2F1), also designated PBR3, RBAP-1, or RBBP-3, is a member of the E2F family of transcription factors.
• E2F1 binds DNA cooperatively with DP proteins through the E2 recognition site (5'-TTTC[CG]CGC-3') found in promoters of genes involved in cell cycle regulation and DNA replication. The DRTF1/E2F complex controls G1-to-S phase transition, and E2F1 binds RB1 in a cell-cycle dependent manner. Related references: PMID:10675335 PMID:12717439 PMID:17050006 PMID:17704056
• It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Related references: PMID:8170954
• Blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Related references: PMID:20176812
• Directly activates transcription of PEG10, and positively regulates transcription of RRP1B. Related references: PMID:17050006 PMID:18625225 PMID:28992046 PMID:20040599
• Subcellular localization: Nucleus.
• Post-translational modifications include acetylation, phosphorylation, methylation, and ubiquitination; also involved in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 301-437) of human E2F1; consider its suitability for detecting full-length E2F1 or specific isoforms.
• For Western blotting, validate detection in nuclear extracts from human, mouse, or rat samples, given the antibody's cross-reactivity.
• For ELISA, the polyclonal nature may be advantageous for capture or detection in sandwich assays; optimize coating concentrations and incubation conditions.
• Since E2F1 is a transcription factor predominantly localized in the nucleus, enrich nuclear fractions to improve signal intensity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This E2F1 polyclonal antibody supports cell cycle and apoptosis research applications. Beyond standard catalog products, CamelBio specializes in sourcing rare-target raw materials and offers comprehensive technical services to bridge the gap from concept to clinic.