DYNC1LI2 Rabbit Polyclonal Antibody for WB - O43237

Core Product Specifications and Parameters

Biological Background: DYNC1LI2 Function and Localization

• DYNC1LI2 encodes a light intermediate chain of cytoplasmic dynein 1, a multi-subunit motor protein complex, and exists as multiple splice isoforms including LIC53 and LIC55.
• The protein serves as a non-catalytic accessory subunit that links the dynein motor to diverse cargos and adapter proteins, enabling retrograde movement along microtubules.
• Cytoplasmic dynein 1 mediates intracellular transport of vesicles, organelles, and possibly chromosomes toward the minus end of microtubules.
• Localizes primarily to the cytoplasm and associates with the cytoskeleton, consistent with its role in microtubule-based motility.
• Contains ATP-binding and nucleotide-binding domains, essential for its function within the ATP-dependent dynein complex.
• Underlies regulatory post-translational modifications including phosphorylation and methylation, which may modulate cargo binding and motor activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central fragment (amino acids 328-492) of human DYNC1LI2; this region may detect both major splice isoforms.
• In western blot, anticipate a predominant band near 54 kDa; validate specificity using siRNA knockdown or blocking peptide in the relevant sample system.
• For IHC-P, consider standard antigen retrieval methods and include isotype controls to confirm staining.
• ELISA performance should be assessed using recombinant DYNC1LI2 protein titrations, and cross-reactivity with other dynein subunits should be ruled out.
• Due to the protein’s involvement in transport, examine expression changes under conditions that alter microtubule dynamics.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by offering a one-stop supply of high-quality raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-DYNC1LI2 antibody supports research in cellular trafficking and dynein motor function, bridging fundamental discovery and diagnostic assay creation. Let our expert team assist in sourcing rare targets and accelerating your project from concept to clinic.