DVL2 Rabbit Polyclonal Antibody for IF/ICC, ELISA - O14641

Core Product Specifications and Parameters

Biological Background: DVL2 Function and Localization

• DVL2 is a segment polarity protein dishevelled homolog, also known as DSH homolog 2, encoded by the DVL2 gene (GeneID: 1856). It belongs to the dishevelled family of scaffold proteins.
• Plays a central role in both canonical and non-canonical Wnt signaling by binding to the cytoplasmic C-terminus of frizzled receptors and transducing signals to downstream effectors. Related references: PMID:24616100
• Promotes internalization and degradation of frizzled proteins upon Wnt stimulation, providing negative feedback regulation of pathway activity.
• Localizes to multiple subcellular compartments including the cell membrane, cytoplasm (cytosol), cytoplasmic vesicles, and nucleus, enabling spatiotemporal control of signaling.
• Undergoes key post-translational modifications such as phosphorylation, isopeptide bond formation, and ubiquitination, which regulate its stability and signaling functions.
• Functions as a developmental protein, mediating Wnt-dependent processes in cell fate determination, proliferation, and tissue patterning.
• UniProt keywords: Developmental protein, Wnt signaling pathway, Cell membrane, Cytoplasm, Nucleus, Phosphoprotein, Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 600-700) of human DVL2, which may exhibit less sequence conservation. Cross-reactivity with mouse is confirmed; validate performance in other species if needed.
• For Immunofluorescence/Immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions (e.g., 4% PFA + 0.1% Triton X-100) and titrate antibody concentration for your cell/tissue model.
• In ELISA, use a known standard (e.g., recombinant DVL2 protein) to generate a calibration curve and determine working dilution.
• As a polyclonal antibody, lot-to-lot variability is possible; include appropriate negative and positive controls and consider re-optimization for new lots.
• For mouse model studies, confirm specificity by verifying lack of staining in Dvl2 knockout tissues or using siRNA knockdown.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials, technical services, and consulting support. This DVL2 polyclonal antibody supports research into Wnt signaling pathways, a critical area in developmental biology and oncology. Explore CamelBio’s extensive portfolio, including validated antibody pairs, recombinant proteins, and ancillary reagents, to streamline your assay development from concept to clinic.