DUSP4 Rabbit Polyclonal Antibody for WB, ELISA - Q13115

Core Product Specifications and Parameters

Biological Background: DUSP4 Function and Localization

• DUSP4, also known as MKP-2 or hVH2, is a dual specificity protein phosphatase that belongs to the MAP kinase phosphatase family.
• Its primary function is to regulate mitogenic signal transduction by dephosphorylating both threonine and tyrosine residues on the MAP kinases ERK1 and ERK2, thereby acting as a negative regulator of the MAPK pathway.
• The gene undergoes alternative splicing, giving rise to multiple transcript variants that may encode different protein isoforms.
• DUSP4 is predominantly localized in the nucleus, consistent with its role in modulating nuclear MAPK signaling events.
• Post-translational modifications include acetylation and phosphorylation, which may influence its activity and stability.
• As a hydrolase and protein phosphatase, DUSP4 is involved in the precise control of cellular proliferation and differentiation through the reversible dephosphorylation of key signaling molecules.

Experimental Guidance and Technical Tips

• The immunogen is derived from the N-terminal region (amino acids 1–168) of human DUSP4, which may allow recognition of multiple isoforms if the epitope is conserved.
• For Western blot, the predicted molecular weight is approximately 43 kDa; a specific band near this size should be observed, but validation with appropriate positive and negative controls is recommended.
• In ELISA applications, the recombinant immunogen can be utilized as a positive control to assess antibody specificity and titer.
• Due to the polyclonal nature, this antibody may exhibit batch-to-batch variability; it is advisable to qualify each new lot in the intended assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing streamlined access to high-quality IVD raw materials, including the DUSP4 polyclonal antibody for MAPK pathway studies. Our extensive catalog encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets. With a comprehensive one-stop approach, CamelBio accelerates your development from concept to clinic.