DTL Rabbit Polyclonal Antibody for WB and ELISA - Q9NZJ0

Core Product Specifications and Parameters

Biological Background: DTL Function and Localization

• DTL (denticleless protein homolog), also known as CDT2, RAMP, DCAF2, and L2DTL, is a substrate-specific adapter of the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex.
• It mediates polyubiquitination and subsequent degradation of CDT1, a key regulator of DNA replication licensing, particularly in response to DNA damage. Related references: PMID:16861906 PMID:16949367 PMID:16964240 PMID:17085480
• DTL also targets CDKN1A/p21(CIP1) for degradation during S phase and after UV irradiation, which is essential for proper replication licensing. Related references: PMID:18794348 PMID:19332548
• The complex degrades KMT5A, influencing TGF-beta signaling, cell cycle progression, DNA repair, and cell migration. Related references: PMID:23478445
• DTL promotes PCNA monoubiquitination at Lys-164, facilitating PCNA-dependent translesion DNA synthesis. Related references: PMID:20129063 PMID:23478441 PMID:23478445
• It also regulates circadian rhythm by mediating ubiquitination and degradation of CRY1. Related references: PMID:26431207
• Subcellular localization includes the nucleus, nuclear membrane, cytoplasm, centrosome, and chromosomes.
• DTL is highly expressed in placenta, testis, thymus, and bone marrow, with lower expression in spleen, lymph node, and peripheral leukocytes; its expression is downregulated by retinoic acid in NT2 cells.
• The protein contains WD repeats and undergoes post-translational modifications such as acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 541–730 near the C-terminus, which may detect full-length DTL and possible isoforms. For western blotting, expect a band around 79 kDa.
• Given the tissue expression pattern, consider using placenta, testis, or hematopoietic cell lines as positive controls for WB.
• For ELISA applications, coat with recombinant protein or use cell lysates; validate with a known standard curve.
• Cross-reactivity with mouse and rat has been reported; verify performance in your specific sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. Our DTL polyclonal antibody supports research in cell cycle regulation, DNA damage response, and ubiquitin-mediated proteolysis. Contact us for bulk orders or assistance in sourcing rare-target antibodies and diagnostic-grade components.