DRD2 Rabbit Polyclonal Antibody for WB and ELISA - P14416

Core Product Specifications and Parameters

Biological Background: Dopamine D2 Receptor Function and Localization

• D(2) dopamine receptor (DRD2) is a G protein-coupled receptor that inhibits adenylyl cyclase activity, reducing intracellular cAMP levels. Related references: PMID:21645528 PMID:39103320
• DRD2 undergoes alternative splicing, generating isoforms with distinct functional properties.
• It positively regulates postnatal regression of retinal hyaloid vessels by suppressing VEGFR2/KDR activity, downstream of the photoreceptor OPN5.
• DRD2 localizes to the cell membrane and the Golgi apparatus membrane.
• The receptor is expressed in the anterior pituitary gland.
• Key post-translational modifications include glycosylation, palmitoylation, and formation of intramolecular disulfide bonds.
• DRD2 is a transmembrane protein with seven transmembrane helices, belonging to the class A rhodopsin-like GPCR family.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human DRD2 (NP_000786.1). This N-terminal region may generate antibodies recognizing both glycosylated and unglycosylated forms.
• For Western blot, the predicted molecular weight is ~51 kDa; however, post-translational modifications may cause band shifts.
• Validate antibody specificity in your model system by using appropriate controls (e.g., knockdown, blocking peptide).
• This polyclonal antibody has reported cross-reactivity with mouse and rat DRD2; sequence alignment of the immunogen region can help predict reactivity with other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents for dopamine receptor research. Our platform connects diagnostic developers with high-quality polyclonal antibodies like this DRD2 pAb, facilitating streamlined procurement from discovery to commercialization. Partner with CamelBio to accelerate your assay development with reliable, tailored raw materials.