DPP3 Rabbit Polyclonal Antibody for WB, ELISA - Q9NY33

Core Product Specifications and Parameters

Biological Background: DPP3 Function and Localization

• DPP3, also known as dipeptidyl peptidase III or enkephalinase B, is a zinc-dependent metalloprotease belonging to the M49 family of peptidases.
• It cleaves and degrades bioactive peptides, including angiotensins, Leu-enkephalin, and Met-enkephalin, modulating their physiological roles. Related references: PMID:1515063 PMID:3233187
• In vitro, DPP3 can hydrolyze the synthetic substrate Arg-Arg-β-naphthylamide. Related references: PMID:11209758 PMID:3233187 PMID:9425109
• DPP3 localizes predominantly to the cytosol, consistent with its role in intracellular peptide turnover.
• At the protein level, DPP3 has been detected in placenta and erythrocytes, suggesting tissue-specific functions in these sites.
• The protein undergoes post-translational acetylation, and alternative splicing generates multiple isoforms.
• Its three-dimensional structure has been solved (3D-structure keyword), facilitating structure-function studies.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1–280) suggests the antibody may recognize the N-terminal region; consider using appropriate blocking peptides or epitope mapping if needed.
• For WB, the predicted molecular weight is ~83 kDa; ensure complete denaturation and reduce samples to avoid aggregation.
• For ELISA, validate the antibody using recombinant DPP3 or relevant biological samples (placenta, erythrocytes) to confirm sensitivity.
• Given cross-reactivity with human, mouse, and rat, this antibody can be used for comparative studies across species.
• As a polyclonal antibody, lot-to-lot variability may occur; consider pre-testing in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, including this DPP3 polyclonal antibody. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support your peptide metabolism and protease research from concept to clinic.