Dopamine Transporter/DAT Rabbit mAb for WB, IHC-P, ELISA - Q01959

Core Product Specifications and Parameters

Biological Background: Dopamine Transporter Function and Localization

• The dopamine transporter (DAT), also known as solute carrier family 6 member 3 (SLC6A3/DAT1), is a sodium- and chloride-dependent neurotransmitter transporter belonging to the sodium:neurotransmitter symporter family.
• It mediates the reuptake of dopamine from the synaptic cleft, critically regulating dopaminergic signaling (PubMed:10375632, PubMed:11093780, PubMed:1406597).
  Related references:
  PMID:10375632
  PMID:11093780
  PMID:1406597
• DAT also transports norepinephrine with lower affinity, a function inferred from similarity to other SLC6 family members.
• In the retina, DAT is involved in light-dependent regression of hyaloid vessels, acting downstream of the OPN5 photoreceptor, as suggested by similarity data.
• The protein localizes to the cell membrane, specifically enriched in neuron projections and axonal compartments, consistent with its role in presynaptic dopamine reuptake.
• It is highly expressed in dopaminergic brain regions: substantia nigra (PubMed:7637582) and striatum, where it is found in axonal varicosities of nerve terminals (PubMed:17296554).
  Related references:
  PMID:7637582
  PMID:17296554
• DAT is a glycoprotein with predicted disulfide bonds and metal-binding sites; it spans the membrane 12 times. Mutations and dysregulation are associated with various neurological disorders, including Parkinsonism, dystonia, and neurodegeneration.

Experimental Guidance and Technical Tips

• The antibody was generated against a synthetic peptide immunogen; verify epitope accessibility in your specific assay format and sample preparation.
• For Western blotting (WB), a band around 68 kDa is expected; consider using reducing and denaturing conditions to fully linearize this multi-pass membrane protein.
• In immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval may be necessary; test standard heat-induced epitope retrieval protocols.
• When using ELISA, the monoclonal format makes this antibody suitable as a capture or detection reagent; perform appropriate pairing and dilution optimization experiments.
• As the antibody shows cross-reactivity with mouse and rat, validate performance in your tissue or cell system of interest before large-scale studies.

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