DNMT3A Rabbit pAb for WB, IP, ELISA - Q9Y6K1

Core Product Specifications and Parameters

Biological Background: DNMT3A Function and Localization

• DNMT3A is required for genome-wide de novo DNA methylation and is essential for establishing DNA methylation patterns during development. It modifies DNA in a non-processive manner and also methylates non-CpG sites. DNA methylation is coordinated with methylation of histones. Related references: PMID:12138111 PMID:16357870 PMID:30478443
• May preferentially methylate DNA linker between two nucleosomal cores and is inhibited by histone H1 (By similarity).
• Plays a role in paternal and maternal imprinting and is required for methylation of most imprinted loci in germ cells (By similarity).
• Acts as a transcriptional corepressor for ZBTB18, recruits HDAC activity, and is recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (By similarity).
• Exhibits weak auto-methylation activity on Cys-710 in the absence of DNA (By similarity).
• Subcellular localization: predominantly nuclear, associated with chromosomes, and also detected in the cytoplasm.
• Tissue expression: highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, and kidney; lower expression in placenta, brain, liver, colon, spleen, small intestine, and lung.
• Alternative promoter usage and alternative splicing generate multiple isoforms, including the shorter DNMT3A2. The protein contains zinc-finger domains, a methyltransferase domain, and is subject to phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–132) of human DNMT3A. Consider that isoforms lacking this region (e.g., certain splice variants) may not be detected.
• For Western blot, the predicted molecular weight of the full-length protein is ~102 kDa. Validate the observed bands using positive controls or recombinant protein; be aware of potential isoform variations.
• For immunoprecipitation, optimize the antibody concentration and incubation conditions with your specific sample type. Nuclear extraction protocols are recommended due to the predominant nuclear localization of DNMT3A.
• The antibody shows cross-reactivity with mouse and rat DNMT3A. When targeting other species, verify sequence homology in the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. Our comprehensive portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target custom sourcing. For epigenetic targets such as DNMT3A, we provide reliable access to critical reagents and technical consultation to streamline your de novo methylation research and assay development.