DNMT1 Rabbit pAb for WB, IF/ICC, IP, ELISA - P26358

Core Product Specifications and Parameters

Biological Background: DNMT1 Function and Localization

• DNMT1 (DNA (cytosine-5)-methyltransferase 1) is the primary maintenance methyltransferase that copies DNA methylation patterns to the newly synthesized strand during DNA replication, ensuring epigenetic inheritance (PubMed:17200670, PubMed:21745816). Related references: PMID:17200670, PMID:21745816

• DNMT1 preferentially methylates hemimethylated CpG dinucleotides and is essential for the faithful propagation of methylation patterns during cell division (PubMed:21745816, PubMed:26070743). Related references: PMID:21745816, PMID:26070743

• Beyond replication-coupled maintenance, DNMT1 associates with chromatin during G2 and M phases to sustain DNA methylation independently of replication (PubMed:21745816). Related references: PMID:21745816

• DNMT1 mediates transcriptional repression directly through interaction with HDAC2 and coordinates DNA methylation with histone modifications (PubMed:10888872, PubMed:16357870). Related references: PMID:10888872, PMID:16357870

• In colorectal cancer cells, DNMT1 forms a corepressor complex that mediates oncogenic KRAS-driven promoter hypermethylation and silencing of tumor suppressor genes (PubMed:24623306). It also maintains a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (PubMed:24623306). Related references: PMID:24623306

• DNMT1 is ubiquitously expressed, with highest levels in fetal tissues, heart, kidney, placenta, and peripheral blood mononuclear cells, and lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less abundant than isoform 1.

• DNMT1 contains a CXXC-type zinc finger domain and undergoes regulatory post-translational modifications such as acetylation, phosphorylation, and methylation.

• DNMT1 localizes to the nucleus and associates with chromosomes, consistent with its role in maintenance methylation and transcriptional regulation.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 760-1109) of human DNMT1, which encompasses the catalytic methyltransferase domain; this design targets the active site and may enhance detection of full-length DNMT1.
• For western blotting, expect a band at approximately 183 kDa; using a low-percentage (e.g., 6–8%) SDS-PAGE gel and extended transfer times can help resolve this high-molecular-weight protein.
• DNMT1 expression levels vary across cell types and tissues; validate antibody performance in your specific sample system using appropriate positive (e.g., HeLa, HEK293) and negative controls.
• For immunoprecipitation, optimize antibody concentration and consider cross-linking to beads to reduce IgG heavy chain interference at ~50 kDa.
• In immunofluorescence/ICC, nuclear permeabilization (e.g., with Triton X-100) and appropriate fixation are required for nuclear antigen access; co-staining with a nuclear marker may confirm localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutes with seamless access to premium IVD raw materials. This DNMT1 polyclonal antibody is part of a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target sourcing for epigenetics and oncology research. Reach out to explore how CamelBio’s technical service and consulting offerings can support your project from concept to clinic.