DNAJA4 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q8WW22

Core Product Specifications and Parameters

Biological Background: DNAJA4 Function and Localization

• DNAJA4 (DnaJ homolog subfamily A member 4) is encoded by the DNAJA4 gene and is also known as MST104, MSTP104, or PRO1472.
• It belongs to the DnaJ/Hsp40 chaperone family and contains zinc-finger domains, suggesting a role in protein folding and metal ion binding.
• The protein is membrane-associated (subcellular location: Membrane) and is prenylated, indicating post-translational lipid modification for membrane anchoring.
• Alternative splicing generates multiple isoforms, expanding functional diversity.
• Post-translational modifications include methylation and phosphorylation, which may regulate activity.
• It has a predicted molecular weight of approximately 44.8 kDa (UniProt) and migrates at 45 kDa on SDS-PAGE (antibody validation).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300-397 of human DNAJA4, a region that may be conserved across species, supporting cross-reactivity with mouse and rat.
• For Western blot (WB), use standard conditions and consider optimizing detection of the 45 kDa band in whole-cell lysates or membrane fractions.
• In immunoprecipitation (IP), validate the antibody in your specific sample system; co-IP may reveal interacting chaperone partners.
• ELISA performance should be confirmed by coating with recombinant protein or cell lysates under native/denaturing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials for chaperone and membrane protein research. We offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to support your DNAJA4-related projects from discovery to clinical application. Our sourcing expertise ensures reliable supply of rare-target raw materials.