DMP1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q13316

Core Product Specifications and Parameters

Biological Background: Dentin Matrix Acidic Phosphoprotein 1 (DMP1) Function and Localization

• DMP1, also known as ARHP/ARHR, is a dentin matrix acidic phosphoprotein 1 encoded by the DMP1 gene in humans.
• It exhibits a dual function depending on phosphorylation state and localization: in the nucleus of undifferentiated osteoblasts, unphosphorylated DMP1 acts as a transcriptional regulator for osteoblast-specific genes such as osteocalcin; upon phosphorylation, it is exported to the extracellular matrix to regulate hydroxyapatite nucleation.
• DMP1 is expressed in tooth, particularly in odontoblast, ameloblast, and cementoblast cells, indicating a key role in dentinogenesis and enamel formation.
• The protein shuttles between multiple subcellular compartments, including the nucleus, cytoplasm, and is also secreted into the extracellular matrix and space, reflecting its dynamic functional states.
• Post-translational modifications, notably phosphorylation, are critical for its functional switch from a nuclear transcription factor to an extracellular matrix mineralization regulator.
• DMP1 is classified under keywords such as biomineralization, phosphoprotein, glycoprotein, and alternative splicing, highlighting its importance in mineralized tissue formation and regulation.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to a peptide within amino acids 413-513 of human DMP1, which is located in the C-terminal region and may help detect both the full-length and processed forms. Validate in your specific sample system.
• This monoclonal antibody is suitable for WB, IF/ICC, and ELISA. For IF/ICC, consider the dual nuclear and cytoplasmic localization of DMP1 depending on cell type and differentiation state.
• In western blotting, a band around 56 kDa is expected, but alternative splicing or post-translational modifications may result in additional bands; confirm with appropriate controls.
• Given its role in osteoblast differentiation, this antibody may be useful in studies involving bone or tooth mineralization models. Include proper positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement, offering validated monoclonal and polyclonal antibodies, including this DMP1 monoclonal antibody, along with ancillary reagents for bone and dental tissue research. Our one-stop platform supports diagnostic manufacturers and labs from concept to clinic, ensuring reliable access to high-quality components and rare target materials.