DMGDH Rabbit mAb for WB, IF-P, ELISA - Q9UI17

Core Product Specifications and Parameters

Biological Background: DMGDH Function and Localization

• DMGDH (dimethylglycine dehydrogenase, mitochondrial) is a mitochondrial enzyme that catalyzes the oxidative demethylation of N,N-dimethylglycine to produce sarcosine, playing a key role in choline and one-carbon metabolism.
• The enzyme also exhibits low-level activity with sarcosine as a substrate in vitro, indicating potential substrate flexibility.
• DMGDH is a flavoprotein containing flavin adenine dinucleotide (FAD) as a cofactor and belongs to the oxidoreductase family.
• The protein is synthesized as a precursor with a mitochondrial transit peptide that directs its import into the mitochondrial matrix, where it exerts its catalytic function.
• Alternative splicing results in multiple transcript variants, and the protein is subject to acetylation and proteomic identification, reflecting its dynamic post-translational regulation.
• Gene sequence variation in DMGDH has been associated with disease phenotypes (keyword: Disease variant), highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 472-866) of human DMGDH, which may provide good specificity across species due to conserved epitopes.
• For Western blotting, the full-length DMGDH protein has a predicted molecular weight of ~97 kDa; a smaller ~45 kDa immunoreactive band may also be detected, possibly representing a processed or degradation form. Validate in the relevant sample system.
• In immunofluorescence, a mitochondrial distribution is anticipated; co-staining with mitochondrial markers is recommended.
• The antibody is reactive with human, mouse, and rat, making it suitable for cross-species studies; however, verify expression levels in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering a comprehensive portfolio that includes antibodies, antigens, and ancillary reagents. This DMGDH Rabbit mAb supports research in mitochondrial metabolism and biomarker development. We provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk sourcing solutions for diagnostic manufacturing, backed by expert technical consulting to accelerate your project from concept to clinic.