DLX5 Rabbit mAb for WB, ELISA - P56178

Core Product Specifications and Parameters

Biological Background: DLX5 Function and Localization

• DLX5 (Homeobox protein DLX-5) is a transcription factor that acts as an immediate early BMP-responsive activator, essential for osteoblast differentiation.
• It stimulates the promoter activity of ALPL and SP7 in a RUNX2-independent manner, promoting osteoblast differentiation markers.
• DLX5 positively regulates chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton, contributing to skeletal development.
• The protein localizes to the nucleus and binds DNA via a 5'-TAATTA-3' consensus sequence.
• DLX5 is subject to alternative splicing and is a phosphoprotein, suggesting regulatory post-translational modifications.
• Mutations in DLX5 are associated with split-hand/foot malformation and deafness, highlighting its role in limb and ear development.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the central region (aa 150-250) of human DLX5, which may allow recognition of full-length and certain isoforms. Validate isoform specificity using relevant cell lysates.
• For Western blot, use positive controls such as osteoblast cell lines (e.g., Saos-2 or U2OS) stimulated with BMP to induce DLX5 expression.
• In ELISA applications, consider coating with the immunizing peptide or recombinant DLX5 protein, and optimize antibody concentration for linear detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our DLX5 antibody supports research in bone development, osteoblast differentiation, and transcriptional regulation, offering a reliable tool for your diagnostic and assay development needs. With expertise in sourcing rare targets and custom immunogen design, CamelBio bridges the gap from discovery to clinical application.