DLGAP5 Rabbit pAb for WB, IF/ICC, ELISA - Q15398

Core Product Specifications and Parameters

Biological Background: DLGAP5 (Disks large-associated protein 5) Function and Localization

• DLGAP5, also known as DLG7, HURP (hepatoma up-regulated protein), or discs large homolog 7, is a potential cell cycle regulator implicated in carcinogenesis.
• It functions as a mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway, ensuring timely degradation during cell cycle progression.
• DLGAP5 is a key regulator of adherens junction integrity and differentiation, potentially involved in CDH1-mediated adhesion and signaling in epithelial cells.
• The protein localizes to the nucleus, cytoplasm, and cytoskeleton, specifically associating with the mitotic spindle.
• Tissue expression is highest in fetal liver, with lower levels in bone marrow, testis, colon, and placenta.
• Post-translational modifications include phosphorylation, isopeptide bond formation (ubiquitination), and Ubl conjugation, with its 3D structure solved; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 547–846, a region that may be present in multiple DLGAP5 isoforms; confirm isoform recognition in your sample type.
• For Western blot, expect a band near 95 kDa in human cell lysates; validate using positive controls such as fetal liver or cancer cell lines.
• In IF/ICC, anticipate spindle-associated cytoplasmic and nuclear staining in mitotic cells; optimize fixation methods to preserve antigenicity.
• For ELISA, consider pairing with a capture antibody targeting a distinct epitope to improve specificity; perform cross-reactivity checks with related family members if needed.

CamelBio: Your One-Stop Sourcing Bridge

This DLGAP5 polyclonal antibody supports cell cycle and oncology research, and CamelBio facilitates seamless procurement of this reagent alongside validated antibody pairs, optimized bulk polyclonal antibodies, and ancillary raw materials. Our one-stop platform bridges diagnostic developers and research institutes to rare-target sourcing, technical consultation, and scalable supply solutions—streamlining your journey from concept to clinic.