DLAT Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q8BMF4

Core Product Specifications and Parameters

Biological Background: DLAT Function and Localization

• The pyruvate dehydrogenase (PDH) complex catalyzes the conversion of pyruvate to acetyl-CoA and CO2, linking glycolysis to the tricarboxylic acid (TCA) cycle.
• DLAT encodes dihydrolipoamide acetyltransferase (E2), the core enzyme that accepts acetyl groups from the E1 component and transfers them to coenzyme A to form acetyl-CoA.
• The PDH complex is composed of multiple copies of E1 (pyruvate dehydrogenase), E2 (dihydrolipoamide acetyltransferase), and E3 (dihydrolipoamide dehydrogenase).
• DLAT localizes to the mitochondrial matrix.
• The protein undergoes lipoylation on specific lysine residues, a modification essential for its catalytic function.
• Acetylation and phosphorylation are key post-translational modifications regulating PDH complex activity.
• The gene is widely expressed, reflecting its central role in cellular energy metabolism.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 68 kDa; use mitochondria-rich tissue or cell lysates (e.g., liver, muscle) as positive controls.
• In immunofluorescence and immunohistochemistry, consider that DLAT is a mitochondrial protein; ensure permeabilization steps are optimized for mitochondrial detection.
• As a polyclonal antibody, lot-to-lot consistency should be validated for quantitative assays, and suitable controls should be included.
• For ELISA applications, pairing with a compatible detection antibody may be necessary; validate in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting. For metabolism-related targets like DLAT, we offer consistent-quality polyclonal antibodies, ancillary reagents, and sourcing support for assay development from concept to clinic.