DISC1 Rabbit mAb for WB, IF/ICC, ELISA - Q9NRI5

Core Product Specifications and Parameters

Biological Background: DISC1 Function and Localization

• DISC1 is a developmental protein involved in multiple aspects of embryonic and adult neurogenesis, including neural progenitor proliferation and newborn neuron integration. Related references: PMID:19303846, PMID:19502360
• It positively regulates Wnt-mediated neural progenitor proliferation by modulating GSK3B activity and CTNNB1 abundance. Related references: PMID:19303846
• DISC1 modulates the AKT-mTOR signaling pathway to control the tempo of neuron positioning, dendritic development, and synapse formation.
• It regulates hippocampal development by controlling the migration of early-born granule cell precursors. Related references: PMID:19502360
• Together with PCNT, DISC1 is involved in microtubule network formation. Related references: PMID:18955030
• Subcellular localization includes cytoplasm, cytoskeleton, centrosome, mitochondria, and postsynaptic density.
• Ubiquitously expressed, with high levels in the dentate gyrus of the hippocampus and temporal/parahippocampal cortices.
• Subject to alternative splicing, isopeptide bond formation, and Ubl conjugation; associated with schizophrenia.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminus (aa 1-100) of human DISC1, suggesting the antibody may recognize epitopes in this region. Consider verifying epitope specificity if needed.
• In Western blot, expect a band near 94 kDa; validate using lysates from brain tissues or neuronal cell lines.
• For immunofluorescence, consider the reported localization to cytoplasm, centrosome, and postsynaptic density; include appropriate subcellular markers.
• Cross-reactivity with mouse and rat enables use in rodent models; confirm epitope conservation by sequence alignment.
• For ELISA, optimize coating conditions and use recombinant protein or peptide as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to validated IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. This DISC1 monoclonal antibody supports neurogenesis and schizophrenia research, and we can supply matched antibody pairs, bulk quantities, and ancillary reagents. For custom or rare-target sourcing needs, our technical team is ready to assist.