DiMethyl-Histone H4-K20 (P62805) Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

Histone H4 is a core component of the nucleosome, where it wraps and compacts DNA into chromatin, limiting DNA accessibility and thereby playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. This regulation is achieved through a complex set of post‑translational modifications known as the histone code, as well as nucleosome remodeling. Related references: UniProt P62805

Histone H4 localizes predominantly to the nucleus and is associated with chromosomes. Acetylation can further influence its subnuclear distribution, for example in step 11 spermatids. Related references: UniProt P62805

A key modification is the dimethylation of lysine 20 (H4K20me2), a critical epigenetic mark involved in chromatin compaction, DNA replication, and the DNA damage response. This antibody was raised against a synthetic peptide specifically dimethylated at K20, enabling targeted recognition of this histone code mark. Related references: PMID:12086618 PMID:15964846 PMID:17967882 PMID:32209475

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody is raised against a synthetic dimethylated peptide around lysine 20 of human histone H4. Consider using the immunizing peptide or a non‑modified peptide as a specificity control in your assays.
• Recommended applications include Western blotting (WB), dot blot (DB), immunohistochemistry‑paraffin (IHC‑P), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. Always optimize antibody dilution and incubation conditions for your specific sample type.
• Histone H4 is highly conserved across eukaryotes; cross‑reactivity has been observed with human, mouse, and rat, and broad reactivity is predicted in other species. Validate in your relevant sample system.
• For methyl‑specific antibodies, aliquot and avoid repeated freeze‑thaw cycles to maintain activity. Use appropriate blocking and washing buffers to preserve the modification‑specific epitope.
• When detecting histone modifications by Western blot, acid extraction or histone enrichment may improve the signal‑to‑noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers working on epigenetic regulation and histone modification, this DiMethyl‑Histone H4‑K20 rabbit monoclonal antibody (SWISS: P62805) exemplifies our commitment to delivering high‑quality, application‑validated antibodies for critical biomarker targets. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw‑material sourcing to support your IVD assay development pipeline.