DiMethyl-Histone H3-K9 Rabbit Monoclonal Antibody (Q16695/P68431) for WB, IHC-P, ELISA, ChIP

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin to regulate access by transcription, replication, repair, and chromosomal stability machineries. Nucleosome remodeling and the dynamic histone code of post-translational modifications orchestrate these processes. Related references: UniProt Q16695/P68431

Histone H3 localizes exclusively to the nucleus and associates with chromosomes, where it serves as a structural and regulatory platform. Related references: UniProt Q16695/P68431

The N-terminal tail of histone H3 undergoes extensive modifications, including acetylation, methylation, phosphorylation, and ubiquitination. Dimethylation of lysine 9 (H3K9me2) is a key repressive epigenetic mark linked to heterochromatin organization and gene silencing. Related references: UniProt Q16695/P68431

Two major isoforms are represented by the SWISS accessions: Q16695 (testis-specific histone H3.1t) and P68431 (ubiquitous histone H3.1 encoded by multiple H3C genes). Both share the conserved K9 residue targeted by this antibody, supporting broad species cross-reactivity. Related references: UniProt Q16695/P68431

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide carrying dimethyl‑K9. Consider verifying that the epitope is not masked in your sample and assess selectivity against mono‑ or trimethylated K9 if required.
• Reported applications include WB, DB, IHC‑P, ELISA, and ChIP. For optimal results, optimize sample lysis/fixation and blocking conditions to preserve the dimethyl mark and reduce background.
• Cross‑reactivity with human, mouse, rat, and other predicted species has been documented. Validation in the pertinent cellular or tissue model is recommended before large‑scale experiments.
• For ChIP, perform pilot experiments with positive/negative control loci to confirm enrichment efficiency. Dot blots can serve as a quick check for methyl‑state specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a complete range of IVD raw materials and technical services. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets such as histone modification‑specific antibodies. Backed by end‑to‑end project support from concept to clinic, CamelBio is your trusted partner for epigenetic and chromatin biology reagent supply.