DiMethyl-Histone H3-K79 Rabbit Polyclonal Antibody (Q16695/P68431) – Validated for WB, IHC-P, ChIP-seq

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, essential for wrapping and compacting DNA into chromatin. This structural organization regulates DNA accessibility and plays a central role in transcription, DNA repair, replication, and chromosomal stability. Related references: UniProt Q16695, UniProt P68431

• Histone H3 exists in multiple variants. The canonical histone H3.1 (encoded by genes such as H3C1–H3C12) is ubiquitously expressed, while the testis-specific variant H3.1t (H3-4) is expressed mainly in testicular cells. Both share high sequence similarity, including the conserved K79 residue. Related references: UniProt Q16695 (tissue specificity), UniProt P68431

• Post-translational modifications (PTMs) on histone H3, such as methylation, acetylation, phosphorylation, and others, constitute the "histone code" that governs chromatin dynamics. Lysine 79 (K79) is a notable site for methylation (mono-, di-, and trimethylation), with dimethylation (H3K79me2) implicated in transcriptional regulation, DNA repair, and telomeric silencing. Related references: For related modifications, see PMID:16267050, PMID:17194708, PMID:16457588

• This antibody was raised against a synthetic peptide carrying dimethylated K79. It is designed to specifically detect the dimethylation state of histone H3 at K79, with broad reactivity predicted across many species due to sequence conservation. Related references: UniProt Q16695, UniProt P68431

• Subcellular localization: Histone H3 is primarily located in the nucleus and on chromosomes, where it forms part of the nucleosome core. Related references: UniProt Q16695, UniProt P68431

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was generated using a synthetic peptide containing dimethylated K79 of human histone H3 (NP_003520.1). For similar modification-specific antibodies, consider using synthetic peptides with defined modifications to ensure high specificity.
• Western blotting (WB): Histone H3 has a predicted molecular weight of ~15 kDa. For best results, use acid-extracted histones or whole-cell lysates, and optimize gel percentage and transfer conditions for low-molecular-weight proteins. Validate in your sample system.
• Immunohistochemistry (IHC-P) and Immunofluorescence (IF/ICC): Antigen retrieval (e.g., citrate buffer) may be required. Test a range of antibody dilutions; note that tissue fixation and embedding can affect epitope accessibility.
• Chromatin Immunoprecipitation (ChIP/ChIP-seq): This antibody is listed for ChIP and ChIP-seq applications. Ensure proper cross-linking (e.g., formaldehyde) and chromatin shearing to ~200–500 bp fragments. Include appropriate positive and negative controls for methylation status.
• ELISA and IP: Standard protocols are applicable. For IP, pre-clearing and using appropriate lysis buffers (e.g., RIPA for histone extraction) may improve signal-to-noise ratio.
• Cross-reactivity: The antibody recognizes human, mouse, and rat histone H3, with a wide range of additional species predicted based on sequence homology. Always verify cross-reactivity in your target species.
• General: As with any polyclonal antibody, consider lot-to-lot variability and revalidate when switching lots. Store as recommended (shipped on ice bag) and avoid multiple freeze-thaw cycles.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing for epigenetic and chromatin research. With this DiMethyl-Histone H3-K79 Rabbit pAb, we are ready to support your diagnostic and analytical assay development with reliable supply and expert guidance.