DiMethyl-Histone H3 (K79) Rabbit Polyclonal Antibody (Q16695/P68431) for WB, ELISA, ChIP

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin. This regulation of DNA accessibility is central to transcription, DNA repair, DNA replication, and chromosomal stability. Related references: UniProt Q16695

• The protein localizes to the nucleus and chromosomes, where it forms the structural backbone of chromatin. Related references: UniProt P68431

• Histone H3 undergoes extensive post-translational modifications, including methylation at multiple lysine residues. Di-methylation at Lysine 79 (H3K79me2) is a specific epigenetic mark implicated in gene regulation and chromatin organization. Related references: PMID:16267050 PMID:17194708

• The H3.1t variant (Q16695) is predominantly expressed in testicular cells, while the canonical H3.1 (P68431) is ubiquitously present. Related references: UniProt Q16695

Experimental Guidance and Technical Tips

• The immunogen is a synthetic dimethylated peptide around K79; consider validating modification specificity through peptide competition assays or dot blot analysis.
• For Western blot, a band around 15 kDa is expected. Use nuclear extraction protocols or acid extraction to enrich histones for optimal detection.
• In ChIP assays, optimize crosslinking and sonication conditions to preserve H3K79me2‑chromatin interactions. Include appropriate positive (e.g., active gene promoters) and negative (heterochromatic) control regions.
• For ELISA, coat plates with the dimethylated peptide or purified nucleosomes; verify antibody binding with methylated versus unmodified peptide controls.
• Cross‑reactivity is predicted across a wide range of species; however, it is advisable to validate in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with a streamlined, one‑stop access to IVD raw materials, supporting every step from concept to clinic. This DiMethyl-Histone H3-K79 antibody is ideal for epigenetic research and assay development, and CamelBio offers comprehensive sourcing capabilities—including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and procurement of rare‑target raw materials—to power your diagnostic pipeline.