DiMethyl-Histone H3-K36 (Q16695 / P68431) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ChIP-seq

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin. This limits DNA accessibility to cellular machineries and thereby plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications, collectively known as the histone code, and nucleosome remodeling.
• The canonical histone H3.1 (UniProt P68431) is encoded by multiple replication-dependent genes (H3C1, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3C10, H3C11, H3C12). The variant H3.1t (UniProt Q16695, gene H3-4), also known as H3t or H3/g, is expressed in testicular cells.
• Dimethylation of Lys36 (conventional numbering; corresponds to Lys37 in the precursor sequence) is a specific post-translational modification on the N-terminal tail of histone H3. This modification contributes to the histone code and is implicated in transcriptional elongation and maintenance of genome integrity. Related references:
  • PMID:15983376
  • PMID:16185088
  • PMID:16267050
  • PMID:16627869
  • PMID:17194708
• Both histone H3.1 and H3.1t localize to the nucleus and chromosome, consistent with their roles in chromatin structure and function.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide containing dimethylated Lys36 of human histone H3, designed to confer high specificity for this modification. For critical applications, consider verifying specificity by peptide competition assays or Western blot analysis of histone extracts with known methylation states.
• In Western blot (WB), a band at approximately 15 kDa is expected. Optimize antibody dilution and validate in the relevant sample system, using appropriate loading controls.
• For chromatin immunoprecipitation (ChIP and ChIP-seq), confirm enrichment of dimethyl-H3K36 at actively transcribed gene bodies or other established genomic targets. Include proper positive and negative control loci to ensure ChIP-grade performance.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), fixation and antigen retrieval methods may affect epitope accessibility; consider testing different conditions to achieve optimal signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including this highly specific anti-dimethyl-Histone H3-K36 (Q16695/P68431) rabbit polyclonal antibody for epigenetic and chromatin research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your entire assay development pipeline. Partner with CamelBio to streamline your transition from concept to clinic with reliable and scalable raw-material solutions.