DiMethyl-Histone H3-K14 Rabbit pAb (Q16695/P68431) for WB, IF/ICC, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, the fundamental repeating unit of chromatin. Nucleosomes wrap and compact DNA, thereby limiting DNA accessibility to cellular machineries that require DNA as a template. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications (the "histone code") and nucleosome remodeling.

This antibody recognizes the dimethylated form of lysine 14 (K14) on human histone H3. Two major isoforms are represented by the SWISS accessions Q16695 (Histone H3.1t, expressed in testicular cells) and P68431 (Histone H3.1, a canonical variant). Both isoforms localize to the nucleus and chromosomes.

Lysine 14 is a critical residue within the N-terminal tail of histone H3 that undergoes dynamic post-translational modifications, including acetylation and methylation, which influence chromatin structure and gene expression. Dimethylation of K14 is associated with specific transcriptional outcomes and is part of the broader epigenetic regulatory landscape.

Related references: The core nucleosome function is supported by numerous structural and biochemical studies. For modification-specific insights, see: PMID:19608861, PMID:17194708.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic dimethylated peptide around K14. To ensure dimethyl-specificity, consider validating the antibody against the non-modified K14 peptide or peptides with mono-/tri-methylation at K14.
• For Western blotting, a starting dilution may be optimized using nuclear extract fractions; the expected band is ~15 kDa. Confirm band disappearance in samples treated with K14 demethylase if available.
• In immunofluorescence (IF/ICC), fixation and permeabilization conditions can impact epitope accessibility. It is advisable to test methanol vs. formaldehyde fixation and include a peptide competition control.
• For ELISA, use the immunogen peptide as a positive control and an unmodified K14 peptide as a negative control to verify modification-specific binding.
• The antibody is predicted to cross-react with a wide range of species due to sequence conservation of the histone H3 N-terminal tail. Validate in the species of interest using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers, laboratories, and research institutes with seamless access to critical reagents from concept to clinic. This DiMethyl-Histone H3-K14 Rabbit pAb exemplifies our commitment to delivering quality antibodies for epigenetic research and assay development. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your IVD workflow. Partner with CamelBio to streamline your supply chain and accelerate your diagnostic innovations.